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Creators/Authors contains: "Wang, Jeffrey"

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  1. ; ; ; ; ; (, Proceedings of the 54th ACM Technical Symposium on Computer Science Education)
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  2. ; ; ; ; ; ; ; (, Communications Biology)
    Abstract Using a fluorescence complementation assay, Delivered Complementation in Planta (DCIP), we demonstrate cell-penetrating peptide-mediated cytosolic delivery of peptides and recombinant proteins in Nicotiana benthamiana. We show that DCIP enables quantitative measurement of protein delivery efficiency and enables functional screening of cell-penetrating peptides for in-planta protein delivery. Finally, we demonstrate that DCIP detects cell-penetrating peptide-mediated delivery of recombinantly expressed proteins such as mCherry and Lifeact into intact leaves. We also demonstrate delivery of a recombinant plant transcription factor, WUSCHEL (AtWUS), into N. benthamiana. RT-qPCR analysis of AtWUS delivery in Arabidopsis seedlings also suggests delivered WUS can recapitulate transcriptional changes induced by overexpression of AtWUS. Taken together, our findings demonstrate that DCIP offers a new and powerful tool for interrogating cytosolic delivery of proteins in plants and highlights future avenues for engineering plant physiology. 
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  3. ; ; ; ; ; ; (, IEEE International Conference on Embedded Software Systems (ICESS'22))
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  4. ; ; ; ; ; ; ; ; ; ; et al (, Spine Surgery and Related Research)
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